Clone | DC101-CP132 | ||||||||||||
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Catalog # | CP132 | ||||||||||||
Category | in vivo Recombinant Antibodies | ||||||||||||
Price |
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The DC101-CP132 monoclonal antibody is a chimeric version of the original DC101 antibody. The variable domain sequences are identical to the original DC101 but the constant region sequences have been switched from rat IgG1 to mouse IgG2a. The DC101-CP132 antibody contains no Fc mutations just as the original rat IgG1 antibody does not. The DC101-CP132 monoclonal antibody reacts with mouse VEGFR-2 (vascular endothelial growth factor receptor 2) also known as CD309, KDR, and Flk-1. VEGFR-2 is a member of the tyrosine protein kinase family. Upon binding to its ligand VEGF, VEGFR-2 pays key roles in vascular development and permeability. VEGFR-2 is expressed on endothelial cells at high levels in adult heart, lung, kidney, brain, and skeletal muscle as well as other tissues at lower levels. The DC101 antibody has been shown to inhibit VEGFR-2 signaling in vivo.
See the references for the original rat IgG1 DC101 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0060).
Isotype | Mouse IgG2a, κ |
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Recommended Isotype Control(s) | InVivoPlus mouse IgG2a isotype control, unknown specificity |
Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
Immunogen | Mouse VEGFR-2-SEAPs soluble receptor |
Reported Applications | in vivo blocking of VEGF/VEGFR-2 signaling* in vitro blocking of VEGFR signaling* Western blot* *Reported for the original rat IgG1 DC101 antibody |
Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
Endotoxin | <1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay |
Aggregation | <5% Determined by DLS |
Purity | >95% Determined by SDS-PAGE |
Sterility | 0.2 µm filtration |
Production | Purified from CHO cell supernatant in an animal free facility |
Purification | Protein G |
Molecular Weight | 150 kDa |
Murine Pathogen Tests | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |