Orders are sent by Federal Express Priority overnight service for shipments processed within the United States. Orders are processed for shipment Monday through Thursday with a cut-off time for shipping at 2:00 p.m. Orders are NOT processed for shipments on Fridays as there is no guarantee that the product can be received and stored properly.

There are three levels of shipping costs:

$70.00 standard

$25.00 for all orders under $200.00

$22.00 handling fee if shipping collects via an internal Federal Express account number provided on the order.

*The shipping costs do not change if there are multiple products being ordered.*

We do not offer free or trial sized samples for testing purposes.

First, please fill out and submit a cell line transfer sheet which can be found here. Then ship the cells frozen and packed with plenty of dry ice via FEDEX overnight delivery. Please do not ship on Thursdays. Make sure to put the “Dry Ice” label (black diamond) on the box.

Store your antibodies undiluted at 4°C in the dark. We do not recommend freezing antibodies (even when aliquoted) as this can result in a loss of activity caused by the freezing/thawing process. Additionally, diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Proteins, in general, are less susceptible to degradation when stored at higher concentrations. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer (just make sure to keep the antibody sterile).

Bio X Cell’s products will remain stable for 1 year from the date of receipt if the antibodies are stored under the conditions outlined on our product data sheets.

Most of our antibodies are supplied as an aqueous solution in PBS containing 20mM NaH2PO4/KHPO4 and 135mM NaCl. However, we can supply antibodies in other buffers when requested. The pH will vary depending on the pI of the individual antibody. The buffer will not contain preservatives such as azide or protein stabilizers such as BSA.

The concentration of our products varies from lot to lot but is typically between 4 and 10 mg/ml. The exact concentration will be marked on the tube itself. If you need to know the exact concentration of a product before ordering, please contact tech@bxcell.co.kr.

Our antibodies are produced using standard hybridoma technology and so the AA or DNA sequence of the antibody is unknown.

We have not epitope mapped our antibodies at Bio X Cell. Searching the published literature would be the best way to determine if this information is available.

We recommend diluting our antibodies in the InVivoPure dilution buffer matching the pH that the antibody is supplied in. In the absence of InVivoPure dilution buffer sterile 1x PBS matching the exact pH of the antibody can be used. It is important to match the pH of the dilution buffer to the antibody to avoid aggregation.

When diluting we recommend using cold buffer and maintaining sterility by working in a biological safety cabinet and using sterile pipet tips, tubes, syringes, and buffers.

Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided.

We do not have direct experimental data generated at Bio X Cell concerning the half-life of our antibodies in vivo. Antibody half-life can be highly variable and influenced by a variety of factors including species, isotype, antigen distribution, antigen concentration and many others. We recommend reading through the literature associated with the antibody you’re interested in to find published data in an experimental system similar to yours as a first step in estimating the half-life.

The optimal dose and frequency of administration for any given experiment and antibody can vary greatly based on many details including the experimental system (i.e. mouse strain, disease model, etc.), the duration of the experiment, the target tissue, the antigen concentration, and much more. For this reason, the optimal dose is best determined by reading through the product references associated with the antibody you’re interested in to find published data in an experimental system similar to yours. Using this as a starting point you can then optimize the dose and frequency of administration experimentally.

Each of our products has an up-to-date reference list available on the products webpage.

Please see our InVivoPlus Difference page

Environmental conditions such as temperature variations, freezing/thawing, shaking during transport or long term storage might lead to protein aggregation. These aggregates are either settled in the conical tip of the vial or are floating freely in the suspension. These aggregates can be removed by gentle shaking at room temperature; they can also be removed by filtration or centrifugation without significant loss.